ESBL (Extended Spectrum Beta-Lactamase)

Specimen Collection Type

24-Hour Urine

Specimen Preparation

Specification after draw

Transport: The sample should be transported according to the specific laboratory’s instructions to ensure the organism remains viable. This may include refrigerated transport for some specimens or prompt delivery to the lab.

Labeling: The container must be properly labeled with patient identification and specimen source information.

Stability

Frozen Stability: (1 year)

Testing Location

(RML)

Interferences

Interference

Potential interference: Substances that could interfere with the test are handled by the laboratory. Common interferences can come from the presence of AmpC beta-lactamases, which can give false-positive results.

Laboratory handling: Laboratories use specific methods and controls to differentiate between ESBL and AmpC production and ensure accurate results.

Misc Instructions

Miscellaneous instructions to collector

Specimen type: Only pure cultures are suitable for confirmatory ESBL testing. Initial patient samples (e.g., urine, blood) cannot be used directly for ESBL confirmation.

Requisition: The correct laboratory requisition must be submitted with the specimen.

No minimum volume: For this specific confirmatory test, a minimum volume is not applicable as it is performed on isolated bacterial cultures, not directly on patient samples.

Infection prevention: Collect samples using proper aseptic techniques and universal precautions to prevent contamination and the spread of infection.

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