EDTA (lavender top) tube
Specification After Draw (Handling/Processing)
Inversion: Gently invert tubes containing additives (like EDTA or Sodium Citrate) several times immediately after collection to ensure proper mixing of the anticoagulant and prevent clotting.
Centrifugation (if required): If the test requires plasma or serum, the sample may need to be centrifuged. This process must occur within a specific timeframe (e.g., within 4 hours of collection to obtain platelet-poor plasma). The separated plasma or serum must then be transferred to a separate labeled polypropylene tube.
Temperature/Storage:
*Most blood panels can be transported at room temperature for initial handling.
*If testing is delayed, the sample may need to be refrigerated or frozen (and transported on dry ice).
Follow the lab’s specific instructions immediately after the draw to maintain sample integrity.
REFRIGERATED
Molecular
(RML)
“Candida spp
Candida parapsilosis
Panel
Interference
Interference with the test results can be caused by various factors:
Hemolysis: The rupture of red blood cells, often caused by improper collection technique (e.g., using too small a needle, shaking the tube vigorously). Hemolysis can interfere with many assays.
Incorrect tube type: Using the wrong anticoagulant can make the sample unusable.
Improper blood volume: For tubes with a specific blood-to-additive ratio (like sodium citrate tubes), an incorrect volume will lead to inaccurate results.
Patient factors: Certain medications, diet, or underlying conditions can affect the levels of markers being tested (e.g., certain cancer markers can be elevated by non-cancerous conditions).
Contamination: Ensure sterile technique is used to avoid contamination, especially for procedures like biopsies or lumbar punctures.
Miscellaneous Instruction to Collector
Follow Sterile Procedure: Use appropriate personal protective equipment (gloves, lab coat, safety glasses) and use needles/lancets only once, disposing of them in a sharps container.
Patient Identification and Labeling: Properly identify the patient and label all primary containers securely with the required information (patient name, date, time of collection, collector’s initials) immediately after the draw.
Documentation: Carefully complete all necessary forms (e.g., chain of custody forms).
Transport: Place primary containers in a tightly closed, leak-proof secondary container (e.g., a biohazard bag or box) for transport to the lab.
Communication: If a “”problem”” collection occurs (e.g., difficulty obtaining a sample, patient refusal), the collector must thoroughly document the circumstances and notify the appropriate personnel (e.g., the ordering physician or lab contact).
