Mycobacterium tuberculosis Complex Detection and Rifampin Resistance (PCR), Acid

Specimen Container

Sterile, leak-proof container

Specimen Collection Type

Lower respiratory material: sputum, BAL, bronchial brushing, wash or biopsy

Minimum Volume To Collect (Ml)

3 mL

Stability

Ambient Stability: Unable
Refrig Stability: 24 hrs
Frozen Stability: Unable

Preferred Transportation Temperature

REFRIGERATED

Reported

43 – 56 days

Methodology

Nucleic Acid Amplification (NAA), nested real-time polymerase chain reaction (PCR); concentrated specimen with broth-based and/or agar-based culture.

Lab Department

Core

Testing Location

LCA

Test OR Panel

Test

Misc Instructions

Specimen processing (i.e., N-acetyl-L-cystine-sodium hydroxide treatment or equivalent, concentration, grinding, both or neither), mycobacterial culture, and smear when appropriate (smears are not performed on blood or when there is less than 2 mL of fluid). Identification by DNA probes or sequencing will be performed at an additional charge. This culture will often detect Nocardia species and other aerobic actinomyces, and identification appropriate for these organisms will be included.

Unit of Measurement

87116

CPT Codes

14556-5

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