Remarks

All Light Blue (3.2% Sodium Citrate) tubes must be filled to the proper level as indicated on vacutainer tube no matter what size tube you are using

CLSI guideline for centrifugation of platelet poor plasma is 1500 x g for 15 minutes. Centrifuges which have been validated to produce platelet poor plasma are also acceptable (at the validated speed and time)

Platelet poor plasma has a platelet count of less than 10,000 platelets per microliter