Blue
Plasma
1.8 mL
(1.8 mL)
Hemolysis,
Over-filled or under-filled tubes
Capillary draw
AMBIENT
All Light Blue (3.2% Sodium Citrate) tubes must be filled to the proper level as indicated on vacutainer tube no matter what size tube you are using
Do not draw above a running heparin line. If a line has ever had heparin in it, it is not suitable for coagulation tests
CLSI guideline for centrifugation of platelet poor plasma is 1500 x g for 15 minutes. Centrifuges which have been validated to produce platelet poor plasma are also acceptable (at the validated speed and time)
“”Platelet poor plasma”” has a platelet count of less than 10,000 platelets per microliter
Sun-Sat
Same day
Clot Formating
Core
(IHC)
INR
Pro Time
Protime
Protime, PT/INR
PT/International Normalized Ratio
Panel
INR, PT
Avoid hemolysis
COLLECTION
Pediatric Collection: One 1.8 mL Light Blue (3.2% Sodium Citrate) or
One Greiner MiniCollect 0.9 mL Light Blue (3.2% Sodium Citrate) transferred from venous blood
HANDLING
Send unopened whole tube at room temperature (preferred); do not send whole blood frozen
Frozen platelet-poor plasma may be submitted
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