Remarks

All Light Blue (3.2% Sodium Citrate) tubes must be filled to the proper level as indicated on vacutainer tube no matter what size tube you are using

Do not draw above a running heparin line. If a line has ever had heparin in it, it is not suitable for coagulation tests

CLSI guideline for centrifugation of platelet poor plasma is 1500 x g for 15 minutes. Centrifuges which have been validated to produce platelet poor plasma are also acceptable (at the validated speed and time)

Platelet poor plasma has a platelet count of less than 10,000 platelets per microliter