sterile urine cup
URINE
Secure the lid tightly immediately after collection, being careful not to touch the inside of the container or lid.
Label the container clearly with the patient’s full name, date of birth, date, and time of collection.
Prompt transport to the laboratory is crucial. The specimen should be delivered within one hour of collection for the best accuracy.
Refrigeration is necessary if a delay in transport is unavoidable. The sample can be stored in a refrigerator (around 4 degrees Celsius) for up to 24 hours.
If the collection cup has an integrated transfer device, the collector should use it to transfer the appropriate amount of urine into the preservative tubes (e.g., grey top for culture and sensitivity, red/yellow top for urinalysis). The preservative tubes should be inverted several times to mix the sample with the preservative.
qPCR
Contamination is the primary source of interference, especially from the skin’s natural bacteria, vaginal discharge, or menstrual blood. This can lead to inaccurate results or an overdiagnosis of a UTI. The clean-catch method is designed to minimize this.
Medications (prescription and over-the-counter) and certain dietary supplements can affect urinalysis results.
Improper storage or delayed transport can cause the sample’s composition to change, as urinary casts and cells can disintegrate and bacteria can multiply, leading to inaccurate results.
Insufficient sample volume may prevent a complete analysis
Verify patient identity before beginning the procedure.
Explain the clean-catch procedure thoroughly to the patient, ensuring they understand the steps to clean the genital area and collect a midstream sample.
Wash hands thoroughly and wear gloves during the collection and handling process.
Provide a sterile container and the necessary cleansing wipes.
Instruct the patient to void a small amount of urine into the toilet first, then collect the midstream portion into the container without stopping the flow, and finish voiding into the toilet.
Ensure the patient understands not to touch the inside of the container or lid.
Properly dispose of any excess urine, the collection cup lid (if using a transfer device system), and any used materials according to facility protocols.
Document the procedure and any issues in the patient’s record.
