WH STI Bacterial Fungal

Specimen Container

“Eswab/Amies
Sterile urine collection cup”

Specimen Preparation

Chlamydia & Gonorrhea (Bacterial):

Specification after draw: The sample must be placed immediately into the transport medium/preservative tube and the cap tightly sealed. For urine, the volume must be between the indicated black lines on the tube.

Syphilis (Bacterial):

Specification after draw: Follow the “”order of draw”” for blood collection. Store/transport according to lab guidelines (often refrigerated or room temperature, depending on the test)

Candidiasis (Fungal) / Bacterial Vaginosis (Bacterial):

Specification after draw: Place the swab into the transport media. For fluid/tissue, place in a sterile container with a small amount of saline if needed to prevent drying, but no preservative.

Stability

Methodology

qPCR

Testing Location

(RML)

Test OR Panel

Test

Interferences

Interference

Antimicrobials: Collect specimens before the administration of antimicrobial or antifungal therapy whenever possible, as this can interfere with culture results.

Contamination: Avoid contamination with normal flora from adjacent sites (e.g., vaginal mucosa during endocervical swab collection, or saliva when collecting sputum).

Spermicides/Lubricants/Douches: These preparations can interfere with test results, so patients should be instructed to avoid using them before sample collection.

Improper Transport: Using the incorrect transport media (e.g., M4 media for urine), incorrect container, or delays in transport/processing can lead to overgrowth of contaminants or death of fragile pathogens.

Specimen Quantity: Insufficient sample quantity may lead to inaccurate results.

Misc Instructions

Miscellaneous Instructions to Collector

Aseptic Technique: Observe aseptic technique during collection to prevent contamination.

Labeling: Label all primary specimen containers with at least two patient-specific identifiers (e.g., name and date of birth), specimen type, and date/time of collection at the patient bedside.

Patient Instructions: Provide clear instructions to the patient, especially for self-collected samples (e.g., midstream clean-catch urine, or proper self-swabbing technique).

Transport Conditions: Follow specific temperature guidelines for transport. Most specimens for culture should not be frozen for short-term storage (store at 2-8°C instead), while some molecular tests allow room temperature transport.

Prompt Delivery: Deliver specimens to the lab promptly to ensure maximum recovery of viable organisms.

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